Characterization and antioxidant functions of mycosporine-like amino acids in the cyanobacterium Nostoc sp. R76DM

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• 作者：Rajesh P. Rastogi^a ; ^b ; ^{raj_rastogi@rediffmail.com" class="auth_mail" title="E-mail the corresponding author} ; Ravi R. Sonani^b ; ^{ravi123sonani@gmail.com" class="auth_mail" title="E-mail the corresponding author} ; Datta Madamwar^b ; ^{datta_madamwar@yahoo.com" class="auth_mail" title="E-mail the corresponding author} ; Aran Incharoensakdi^a ; ^{aran.i@chula.ac.th" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Cyanobacteria ; UV radiation ; Mycosporine-like amino acids ; Sunscreen ; Antioxidants ; Stability
• 刊名：Algal Research
• 出版年：2016
• 出版时间：June 2016
• 年：2016
• 卷：16
• 期：Complete
• 页码：110-118
• 全文大小：1647 K

文摘

Mycosporine-like amino acids (MAAs) are considered efficient photoprotectants against high energy UV-photon flux. The present study is intended to probe the occurrence and synthesis of UV-absorbing MAAs and characterization of their induction, stability and free radical scavenging capacity in the cyanobacterium m>Nostocm> sp. R76DM. HPLC-PDA detection, and LC–MS analysis revealed the occurrence of four different MAAs, palythine (λ_max 320 nm, m>mm>/m>zm> 245), m>Asterinam> (λ_max 331 nm, m>mm>/m>zm> 289), m>Porphyram> (λ_max 333 nm, m>mm>/m>zm> 347) and palythene (λ_max 359 nm, m>mm>/m>zm> 285) with retention times 2.9, 4.71, 6.29 and 7.05 min, respectively. Induction of the synthesis of all MAAs was highly noticeable under UV-B radiation. These MAAs were exceedingly resistant to some physico-chemical factors such as UV-B, temperature, pH and a strong oxidizing agent. Total MAAs were extensively evaluated for their antioxidant properties by 2,2-diphenyl-1-picryl-hydrazyl, ferric reducing antioxidant power, superoxide radical scavenging and reducing power assay. MAAs exhibited significant and dose-dependent m>in vitrom> antioxidant and m>in vivom> ROS scavenging potentials. Moreover, the results indicate the vital role of MAAs in life-saving mechanisms of cyanobacteria by virtue of their UV-absorbing/screening and antioxidant function. The results also added up evidence in favor of MAAs for possible use as active ingredients in cosmeceuticals.