Spin-trapping agent α-phenyl N-tert-butylnitrone binds to trypsin and enhances heparin-induced inhibition of amidolytic activity and structural degradation of the enzyme
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- 作者：Finotti ; Paola ; Corvaja ; Carlo ; Pagetta ; Andrea
- 关键词：α ; -Phenyl N-tert-butylnitrone ; Heparin ; Trypsin ; Bovine ; Enzyme inhibition ; Oxidation ; PBN ; α ; -phenyl N-tert-butylnitrone ; BAPNA ; Nα ; -benzoyl-DL-arginine-p-nitroanilide hydrochloride ; STI ; soybean trypsin inhibitor
- 刊名：FEBS Letters
- 出版年：1999
- 出版时间：June 18, 1999
- 年：1999
- 卷：453
- 期：1-2
- 页码：159-163
- 全文大小：280 K
文摘
The effects of heparin on trypsin have recently been demonstrated to involve inhibition of catalytic activity and degradation of the enzyme by means of an oxidative mechanism. The possibility that α-phenyl N-tert-butylnitrone protects heparin-induced radical formation on trypsin was investigated by measuring amidolytic activity and changes in the structure of trypsin in the presence of heparin with and without α-phenyl N-tert-butylnitrone. The results show that α-phenyl N-tert-butylnitrone does not only prevent, but it even significantly enhances effects of heparin on the enzyme. This is due to the unique property of α-phenyl N-tert-butylnitrone, independently of spin-trapping capacity, to modify the trypsin structure by binding irreversibly to the catalytic triad, at sites distinct from those to which heparin binds.