Aldehyde dehydrogenase induction in arsenic-exposed rat bladder epithelium

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• 作者：Ya-Chun Huang^a ; Hsin-Su Yu^b ; ^c ; Chee-Yin Chai^a ; ^d ; ^e ; ^f ; ^{cychai@kmu.edu.tw" class="auth_mail" title="E-mail the corresponding author}
• 关键词：2-DE ; two-dimensional electrophoresis ; ALDH ; aldehyde dehydrogenase ; BFD ; blackfoot disease ; CHAPS ; 3-[(3-cholamidopropyl) dimethyl-ammonio]-1-propanesulfonate] ; CK-1 ; type II cytoskeletal 1 ; CK-10 ; type I cytoskeletal 10 ; CSC ; cancer stem cell ; D.D. water ; distilled deionized water ; DAB ; 3&prime ; 3-diaminobenzidine ; DNA ; deoxyribonucleic acid ; DTT ; dithiothreitol ; eEF-1α ; elongation factor-1 alpha ; hsp27 ; heat shock protein 27 ; IACUC ; Institutional Animal Care and Use Committee ; IEF ; isoelectric
• 刊名：Experimental and Toxicologic Pathology
• 出版年：2016
• 出版时间：January 2016
• 年：2016
• 卷：68
• 期：1
• 页码：61-68
• 全文大小：2007 K

文摘

Arsenic is widely distributed in the environment. Many human cancers, including urothelial carcinoma (UC), show a dose-dependent relationship with arsenic exposure in the south-west coast of Taiwan (also known as the blackfoot disease (BFD) areas). However, the molecular mechanisms of arsenic-mediated UC carcinogenesis has not yet been defined. In vivo study, the rat bladder epithelium were exposed with arsenic for 48 h. The proteins were extracted from untreated and arsenic-treated rat bladder cells and utilized two-dimensional gel electrophoresis and mass spectrometry. Selected peptides were extracted from the gel and identified by quadrupole-time of flight (Q-TOF) Ultima-Micromass spectra. The significantly difference expression of proteins in arsenic-treated groups as compared with untreated groups was confirmed by immunohistochemistry (IHC) and western blotting. We found that thirteen proteins were down-regulated and nine proteins were up-regulated in arsenic-treated rat bladder cells when compared with untreated groups. The IHC and western blotting results confirmed that aldehyde dehydrogenase (ALDH) protein was up-regulated in arsenic-treated rat bladder epithelium. Expression of ALDH protein was significantly higher in UC patients from BFD areas than those from non-BFD areas using IHC (p = 0.018). In conclusion, the ALDH protein expression could be used as molecular markers for arsenic-induced transformation.