Characterization and preparation of oligomeric procyanidins from Litchi chinensis pericarp

详细信息    查看全文

• 作者：Yong Sui^a ; ^b ; Yan zheng^c ; Xiaopeng Li^a ; Shuyi Li^d ; Bijun Xie^a ; Zhida Sun^a ; ^{sunzhida@sina.com" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Litchi pericarp ; Oligomeric procyanidins ; Characterization ; Preparation
• 刊名：Fitoterapia
• 出版年：2016
• 出版时间：July 2016
• 年：2016
• 卷：112
• 期：Complete
• 页码：168-174
• 全文大小：511 K

文摘

The main purpose of this study is to characterize and prepare A-type oligomeric procyanidins from litchi pericarp (Litchi chinensis Baila). The variety of oligomeric procyanidins was characterized by LC–ESI–MS analysis. There were (+)-catechin, (−)-epicatechin, twelve dimers and six trimers of procyanidins were found in litchi pericarp extracts, and A-type procyanidins were much more abundant than B-type procyanidins. The main flavan-3-ol monomer and oligomeric procyanidins in litchi pericarp were (−)-epicatechin, A-type dimers (A1 and A2) and trimer (epicatechin-(4β-8, 2β-O-7)-epicatechin- (4β-8)-epicatechin). Procyanidin A1 (epicatechin-(4β-8, 2β-O-7)-catechin) was identified by NMR in litchi pericarp for the first time. (−)-Epicatechin and oligomeric procyanidins were prepared by the combination of AB-8 column chromatography and Toyopearl HW-40S column chromatography. The results showed that each fraction predominantly owned a single compound and gave a high yield with (−)-epicatechin, A-type dimers (A1 and A2) and trimer, suggesting a useful method to obtain pure (−)-epicatechin and A-type oligomeric procyanidins.