Ubiquitous aspartic proteinase as an actor in the stress response in buckwheat

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• 作者：Gordana S. Timotijević ; Mira Dj Milisavljević ; Svetlana R. Radović ; Miroslav M. Konstantinović ; Vesna R. Maksimović
• 关键词：Abiotic stress ; Aspartic proteinase ; Buckwheat ; Gene expression ; Leader intron
• 刊名：Journal of Plant Physiology
• 出版年：2010
• 出版时间：1 January 2010
• 年：2010
• 卷：167
• 期：1
• 页码：61-68
• 全文大小：387 K

文摘

Summary

The aspartic protease (FeAP9) gene from buckwheat resembles the exon–intron structure characteristic for typical aspartic proteinases, including the presence of the leader intron in the 5′-UTR. RT PCR experiments and gel protein blot analysis indicated that FeAP9 was present in all analyzed organs: developing seeds, seedlings, flowers, leaves, roots and stems. Using Real-time PCR, we found that FeAP9 expression is upregulated in buckwheat leaves under the influence of different abiotic stresses, including dark, drought and UV-B light, as well as wounding and salicylic acid.