Synthesis of the multi-walled carbon nanotubes-COOH/graphene/gold nanoparticles nanocomposite for simple determination of Bilirubin in human blood serum

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• 作者：Qingliang Feng ; Yongling Du ; Chao Zhang ; Zhixiang Zheng ; Fangdi Hu ; Zaihua Wang ; Chunming Wang
• 关键词：Bilirubin ; Carbon materials ; Biosensor ; AuNPs
• 刊名：Sensors and Actuators B: Chemical
• 出版年：2013
• 出版时间：August, 2013
• 年：2013
• 卷：185
• 期：Complete
• 页码：337-344
• 全文大小：1940 K

文摘

In this work, the multi-walled carbon nanotubes-COOH (MWCNTs-COOH)/graphene (GNs)/gold nanoparticles (AuNPs) composite materials were synthesized by in situ growth of AuNPs on the surface of the MWCNTs-COOH/GNs. And then, the Bilirubin oxidase (BUD) was immobilized on the surface of the film to construct a biosensor for determination of Bilirubin. Morphology of the nanomaterials was analyzed by field-emission scanning electron microscopy, high resolution transmission electron microscopy, Brunauer-Emmett-Teller, and X-ray photoelectron spectroscopy. The performance of the biosensor was studied by electrochemical impedance spectroscopy (EIS), cyclic voltammetry (CV) and amperometric response (i-t). It was demonstrated that the nanocomposite materials exhibited enhanced fast electron transfer and excellent biocompatibility. In this assay, the MWCNTs-COOH/GNs/AuNPs composite film based biosensor showed prominently electrocatalytic activity for the detection of Bilirubin with a linear range from 1.33 ¦ÌM to 71.56 ¦ÌM and the low limit of detection (LOD) is 0.34 ¦ÌM based on a signal to noise ratio (S/N = 3). Meanwhile, the apparent Michaelis-Menten constant (K_m) was 64.86 ¦ÌM. In addition, this biosensor exhibited satisfying reproducibility, stability, feasibility and fast responses. These results provide a novel way for exact detection of Bilirubin in biotechnology and clinical diagnosis, and broaden the application of graphene and carbon nanotubes in biomaterials.