Transgenic expression of green fluorescent protein in caprine embryos produced through electroporation-aided sperm-mediated gene transfer
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- 作者：R. Kumar Pramod1 ; Rakesh Kumar1 ; Abhijit Mitra ; drabhijitmitra@gmail.com" class="auth_mail" title="E-mail the corresponding author
- 关键词：AV ; Artificial Vagina ; BSA ; Bovine Serum Albumin ; BSA-FAF ; Bovine Serum Albumin-Fatty Acid Free ; COC ; Cumulus&ndash ; Oocyte Complex ; EGFP ; Enhanced Green Fluorescent Protein ; FITC-PSA ; Fluorescein Isothiocyanate-Pisum Sativum Agglutinin ; IVC ; In Vitro Culture ; IVF ; In Vitro Fertilization ; IVM ; In Vitro Maturation ; mSOF ; Modified Synthetic Oviduct Fluid ; OCM ; Oocyte Collection Medium ; qPCR ; Quantitative Real Time PCR ; SMGT ; Sperm Mediated Gene Transfer ; TALP ; Tyrods Albumin Lactate Pyruvate
- 刊名：Gene
- 出版年：2016
- 出版时间：15 January 2016
- 年：2016
- 卷：576
- 期：1
- 页码：505-511
- 全文大小：756 K
文摘
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We report, for the first time, a protocol of electroporation aided SMGT in goat.
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The protocol showed a higher DNA uptake by sperm with minimum adverse effect.
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There was four folds more DNA uptake by sperm cells under electroporation.
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Eletroporation of sperms did not impede the embryo cleavage rate on IVF.
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We produced transgenic fluorescent goat embryos using electroporation aided SMGT.