Subcapsular sinus macrophages limit dissemination of West Nile virus particles after inoculation but are not essential for the development of West Nile virus-specific T cell responses

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• 作者：Ev ; ro R. Winkelmann ; Douglas G. Widman ; Jingya Xia ; Alison J. Johnson ; Nico van Rooijen ; Peter W. Mason ; Nigel Bourne ; Gregg N. Milligan
• 关键词：West Nile virus ; Single-cycle flavivirus ; RepliVAX ; subcapsular sinus macrophages ; CD8+ T cell
• 刊名：Virology
• 出版年：February, 2014
• 年：2014
• 卷：450-451
• 期：Complete
• 页码：278-289
• 全文大小：1674 K

文摘

Macrophages encounter flaviviruses early after injection by arthropod vectors. Using in vivo imaging of mice inoculated with firefly luciferase-expressing single-cycle flavivirus particles (FLUC-SCFV), we examined the initial dissemination of virus particles in the presence or absence of lymph node (LN)-resident macrophages. Higher luciferase activity, indicating higher SCFV gene expression, was detected in the footpad of macrophage-depleted mice after 24 h post infection (hpi). Moreover, FLUC-SCFV particles disseminated to the spleen within 14 hpi in macrophage-depleted, but not control mice. Although macrophages presented SCFV to na茂ve T cells in vitro, depletion of subcapsular sinus (SCS) macrophages did not alter the magnitude or effector function of the WNV-specific CD8⁺ T cell response. Together, these results indicate that SCS macrophages play a role in limiting the dissemination of SCFV early in infection but are not required for the generation of a polyfunctional WNV-specific CD8⁺ T cell response in the draining LN.