Comparative diagnostic potential of three serological tests for abortive Q fever in goat herds

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• 作者：Elodie Rousset ; Benoit Dur ; Mustapha Berri ; Philippe Dufour ; Myriam Prigent ; Pierre Russo ; Thibault Delcroix ; Anne Touratier ; Annie Rodolakis ; Michel Aubert
• 关键词：Q fever ; Coxiella burnetii ; Goat ; Abortion ; Serological test
• 刊名：Veterinary Microbiology
• 出版年：2007
• 出版时间：6 October 2007
• 年：2007
• 卷：124
• 期：3-4
• 页码：286-297
• 全文大小：218 K

文摘

Performances of an ELISA, an immunofluorescence assay (IFA) and a complement fixation test (CFT) were assessed for detecting antibodies against Coxiella burnetii after Q fever abortions in naturally infected goats. The goal of the study was to provide information useful for veterinary serodiagnosis in regard to categories of goats either experiencing Q fever abortion or not, blood sampling times and recommended cut-offs. The study was conducted on eight goat herds with evidence of C. burnetii abortions. In each herd, at least 5 goats that had aborted and 10 goats prior to parturition or at term were monitored 15, 30 and 60 days (D15, D30, D60) after the onset of Q fever abortion. The overall CFT results distribution did not differ between the two groups of goats and showed poor agreement with the ELISA results. In contrast, the ELISA and IFA results revealed comparable significant differences, but overall the ELISA test was slightly more sensitive than the IFA test. Seroprevalence, according to ELISA and IFA respectively, was higher in the aborting (88 % and 82 % ) than in the non-aborting group (60 % and 50 % ). High levels of serum antibodies were detected in goats post-abortion with an average of 114 % OD using ELISA and a log₁₀(titer) of 2.4 using IFA. Strongly positive ELISA ( % OD > 80) and positive IFA results (log₁₀(titers) > 1.9) were significantly associated with abortion. Sampling on D15 gave the best association with ORs of 10 for ELISA and 6 for IFA. The practical interest of these results is discussed.