Evaluation of methods to extract and quantify lipids from Synechocystis PCC 6803

详细信息    查看全文

• 作者：Jie Sheng ; ^a ; ^{Jie.Sheng@asu.edu} ; Raveender Vannela^a ; Bruce E. Rittmann^a
• 关键词：Biodiesel ; Lipid extraction ; Cyanobacteria ; Synechocystis
• 刊名：Bioresource Technology
• 出版年：2011
• 出版时间：January 2011
• 年：2011
• 卷：102
• 期：2
• 页码：1697-1703
• 全文大小：309 K

文摘

In order to use the photosynthetic cyanobacterium Synechocystis as a source of feedstock for carbon–neutral, nonpetroleum-based diesel fuel, we evaluated several solvents and solvent systems for their ability to extract lipid from wild-type Synechocystis PCC 6803. Chloroform + methanol-based Folch and Bligh & Dyer methods had the highest lipid recoveries. Less toxic solvents, such as methanol and MTBE, or direct trans-esterification of biomass (without pre-extraction step) gave only slightly lower lipid-extraction yields. Ethanol, isopropanol, butanol, hexane, acetic ester, and their combinations were not effective for lipid extraction from Synechocystis (>20 % loss), even though they are widely used for non-polar lipid extraction from other feedstock, including algae. We confirmed the success of chloroform + methanol-based extraction by their penetration of the cell membrane system, higher polarity, and stronger interaction with hydrogen bonds. The less-polar solvents not only had lower lipid yield, but also extracted more non-lipid compounds that require extra purification to remove. We also characterized the fatty-acid profile of Synechocystis PCC 6803: C16:0 (60 % ), C16:1 (9.5 % ), C18:0 (1.2 % ), C18:1 (2 % ), C18:2 (9.8 % ), and C18:3 (16.5 % ).