Characterization of human cytomegalovirus UL146 transcripts

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• 作者：Rong He^a ; Yanping Ma^b ; Ying Qi^b ; Shujuan Jiang^a ; Ning Wang^b ; Mali Li^b ; Yaohua Ji^b ; Zhengrong Sun^b ; Qiang Ruan^b ; ^{ruanq@sj-hospital.org}
• 关键词：HCMV ; human cytomegalovirus ; ORF ; open reading frame ; HELF ; human embryonic lung fibroblast ; PFU ; plaque forming units ; IE ; immediate early ; X-Gal ; 5-bromo-4-chloro-3-indolyl-¦Â-d-galactopyranoside ; IPTG ; isopropyl-¦Â-d-thio-galactoside
• 刊名：Virus Research
• 出版年：2012
• 出版时间：January 2012
• 年：2012
• 卷：163
• 期：1
• 页码：223-228
• 全文大小：373 K

文摘

Background

The genome of human cytomegalovirus (HCMV) has been studied extensively, particularly in the UL/b?region. In this study, transcripts of one of the UL/b?genes, UL146, were identified in 3 HCMV isolates obtained from urine samples of congenitally infected infants.

Methods

Northern blot hybridization, cDNA library screening, and RACE-PCR were used.

Results

All sequences of clones from a cDNA library were about 3225 bp in length with the same 5?and 3?ends. The results were accordant with that analyzed by Northen-blot and RACE-PCR in three HCMV clinical isolates. The transcript initiated from the region upstream of UL146 flanking region and terminated just downstream of UL132 including UL146, UL147, UL147A, UL148, and UL132 ORFs. Treatment of the infected cells with phosphonoacetic acid inhibited its transcription.

Conclusions

UL146 ORF was transcribed with 4 downstream ORFs from UL147 to UL132 at true late kinetics. The transcript of UL146 initiated at 73 nt upstream of UL146 and terminated just downstream of UL132 in the 3 clinical isolates.