Role of 1α,25(OH)2 vitamin D3 on α-[1-14C]MeAIB accumulation in immature rat testis
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- 作者：Danusa Menegaz ; Angela Rosso ; Carine Royer ; Laura D. Leite ; Adair R.S. Santos ; Fá ; tima R.M.B. Silva
- 关键词：1α ; 25(OH)2 vitamin D3 ; Amino acid accumulation ; Mechanism ; Testis
- 刊名：Steroids
- 出版年：2009
- 出版时间：February 2009
- 年：2009
- 卷：74
- 期：2
- 页码：264-269
- 全文大小：445 K
文摘
1,25D3 is critical for the maintenance of normal reproduction since reduced fertility is observed in male rats on a vitamin D-deficient diet. Vitamin D-deficient male rats have incomplete spermatogenesis and degenerative testicular changes. In the present study we have examined the ionic involvement and intracellular messengers of the stimulatory effect of 1,25D3 on amino acid accumulation in immature rat testis. 1,25D3 stimulates amino acid accumulation from 10−12 to 10−6 M by increasing the slope to reach a maximum value at 10−10 M, as compared to the control group. No effect was observed at a lower dose (10−13 M). Time-course showed an increase on amino acid accumulation after 15, 30, and 60 min of incubation with 1,25D3 (10−10 M). 1,25D3 stimulated amino acid accumulation in 11-day-old rat testis but not in testis that were 20 days old. Cycloheximide totally blocked the 1,25D3 action on amino acid accumulation. Furthermore, a localized elevation of cAMP increased the stimulatory effect of 1,25D3 and the blockage of PKA nullified the action of the hormone. In addition, 1,25D3 action on amino acid accumulation was also mediated by ionic pathways, since verapamil and apamine diminished the hormone effect. The stimulatory effect of 1,25D3 on amino acid accumulation is age-dependent and specific to this steroidal hormone since testosterone was not able to change amino acid accumulation in both ages studied. This study provides evidence for a dual effect for 1,25D3, pointing to a genomic effect that can be triggered by PKA, as well as to a rapid response involving Ca2+/K+ channels on the plasma membrane.