Proteasomal activity in skeletal muscle: A matter of assay design, muscle type, and age

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• 作者：Karl-Heinz Strucksberg ; Karthikeyan Tangavelou ; Rolf Schrö ; der ; Christoph S. Clemen
• 关键词：Ubiquitin proteasome system ; Proteasomal activity ; Protein degradation ; Proteasome inhibitor ; Skeletal muscle
• 刊名：Analytical Biochemistry
• 出版年：2010
• 出版时间：15 April 2010
• 年：2010
• 卷：399
• 期：2
• 页码：225-229
• 全文大小：301 K

文摘

The ubiquitin–proteasome system (UPS) is a major degradation system for regulatory and misfolded proteins. UPS function has been implicated to exert a central role in the pathogenesis of various human diseases. Because biochemical analyses are often hampered by the amount of available diseased tissue, we report on the establishment and validation of a luminescence-based proteasomal activity assay applicable to 5-mg quantities of skeletal muscle. We demonstrate that the specific proteasomal activity differs in individual muscle groups and decreases with aging. These findings warrant the use of appropriate controls and a careful interpretation of results in mammalian skeletal muscle pathologies.