Effects of ceftriaxone induced intestinal dysbacteriosis on lymphocytes in different tissues in mice
详细信息 查看全文
- 作者：Xia Luo ; Yanyi Zheng ; Ruyan Wen ; Xiangliang Deng ; Lian Zhou ; zl@gzucm.edu.cn" class="auth_mail" title="E-mail the corresponding author ; Haifeng Liao
- 关键词：GALT ; gut-associated lymphoid tissues ; PPs ; Peyer&rsquo ; s patches ; MLN ; mesenteric lymph node ; DTH ; delayed type hypersensitivity ; MIS ; mucosal immune system ; M cell ; microfold cell ; DC ; dendritic cell ; SPF ; specific pathogen-free ; Con A ; concanavalin A ; LPS ; lipopolysaccharides ; DNFB ; 2 ; 4-dinitro-1-fluorobenzene ; CFSE ; 5 ; 6-carboxy fluorescein diacetate succinimidyl ester ; FBS ; fetal bovine serum ; SCFA ; short-chain fatty acid ; PBS ; phosphate balanced solution ; HE ; hematoxylin and eosin
- 刊名：Immunobiology
- 出版年：2016
- 出版时间：September 2016
- 年：2016
- 卷：221
- 期：9
- 页码：994-1000
- 全文大小：1916 K
文摘
The close relationship between intestinal microflora and immune system has been confirmed, stimulus from intestinal flora plays an important role in the development of the immune system and its dynamic balance. Current research is still inadequate to determine how local bacteria in gut influence the whole body. In this study, influence of ceftriaxone sodium induced intestinal dysbacteriosis on local and overall immune function was investigated. We found that the beneficial bacteria decreased significantly compared with control after oral administration of ceftriaxone; Moreover, the proportion of T cells are higher and B cells are lower in the dysbacteriosis mice, activation and proliferation of T and B cells was decreased significantly in gut-associated lymphoid tissues (GALTs), such as Peyer’s patches (PPs) and mesenteric lymph nodes(MLNs)with ceftriaxone treatment; The secreted sIgA in intestinal was reduced in dysbacteriosis mice than that of control as well. The similar results above are also shown on the spleen. In addition, the delayed type hypersensitivity (DTH) reaction decreased in dysbacteriosis mice. The present data suggested that intestinal microflora had impact on immune system by influencing the proportion and function of lymphocytes in PPS-MLN-spleen.