Early detection of placental inflammation by MRI enabling protection by clinically relevant IL-1Ra administration

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• 作者：Sylvie Girard ; PhD^a ; ^{sylvie.girard@manchester.ac.uk} ; Luc Tremblay ; PhD^b ; Martin Lepage ; PhD^b ; Guillaume Sebire ; MD ; PhD^a
• 关键词：inflammation ; interleukin-1 ; magnetic resonance imaging ; placenta
• 刊名：American Journal of Obstetrics and Gynecology
• 出版年：2012
• 出版时间：April, 2012
• 年：2012
• 卷：206
• 期：4
• 页码：358.e1-358.e9
• 全文大小：3907 K

文摘

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Objective

We studied whether magnetic resonance imaging (MRI) could be used to detect placental inflammation before the detection of irreversible tissue damage. Next, we tested whether this early detection would enable the administration of treatment (ie, interleukin-1 receptor antagonist [IL-1Ra]) in a realistic clinical time after diagnosis.

Study Design

Pregnant rats were injected intraperitoneally with lipopolysaccharide with/without delayed IL-1Ra. MRI was performed at different time after the injection, and placentas were collected for comparison. Placental inflammation was assessed by determination of the levels of inflammatory cytokines.

Results

Placental inflammation was detected by MRI as early as 3 hours after maternal administration of lipopolysaccharide, concomitantly to IL-1¦Â up-regulation. This was observed before any tissue damage, which appeared only at 24 hours after the administration of lipopolysaccharide. Delayed IL-1Ra administration (after MRI diagnosis) protected the placenta, as seen by the preserved tissue integrity and limited macrophages infiltration in the placental parenchyma.

Conclusion

These findings established a noninvasive diagnostic method for early in utero detection of placental inflammation that would allow the administration of placentoprotective intervention within a clinically relevant delay after diagnosis.