Core bead chromatography for preparation of highly pure, infectious respiratory syncytial virus in the negative purification mode
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- 作者：Sophia T. Mundlea ; sophia.mundle@sanofipasteur.com" class="auth_mail" title="E-mail the corresponding author ; Michael Kishkoa ; Rachel Groppoa ; Joshua DiNapolia ; John Hambergera ; Bryan McNeilb ; Harry Kleanthousa ; Mark Parringtona ; Linong Zhanga ; Stephen F. Andersona
- 关键词：Respiratory syncytial virus ; Virus purification ; Core bead chromatography ; Hollow fiber tangential flow filtration
- 刊名：Vaccine
- 出版年：2016
- 出版时间：12 July 2016
- 年：2016
- 卷：34
- 期：32
- 页码：3690-3696
- 全文大小：979 K
文摘
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A purification procedure for respiratory syncytial virus is described.
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Purification is achieved by core bead chromatography followed by tangential flow filtration.
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The purification scheme results in ∼60% recovery of infectious virus titer.
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Purified RSV LAV is immunogenic and protective in vivo.
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Purified virus is 50–200-fold more pure with respect to cellular DNA and protein than the raw feed stream.