A colorimetric method was developed for the determination of Fe(III).
Fe(III) directly oxidized 3,3,′5,5′-tetramethylbenzidine to produce blue solution.
The method benefited the characteristics of low cost, sensitivity and selectivity.
As low as 1.0 μmol/L Fe(III) could be perceived by the naked eyes.
Iron content in milks was successfully determined.