Comparative in vitro analyses of recombinant maize starch synthases SSI, SSIIa, and SSIII reveal direct regulatory interactions and thermosensitivity
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- 作者：Binquan Huanga ; 1 ; Peter L. Keelingb ; Tracie A. Hennen-Bierwagena ; Alan M. Myersa ; ammyers@iastate.edu" class="auth_mail" title="E-mail the corresponding author
- 关键词：Starch synthase ; Starch structure ; Maize ; Endosperm ; Thermosensitivity
- 刊名：Archives of Biochemistry and Biophysics
- 出版年：2016
- 出版时间：15 April 2016
- 年：2016
- 卷：596
- 期：Complete
- 页码：63-72
- 全文大小：1413 K
文摘
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SSI and SSIII mixed in a reconstituted system exhibited a negative regulatory interaction.
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The reconstituted system recapitulated the in vivo effects of SSIII mutation.
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SS activities were stimulated by glucans in various buffers, upholding the accepted mechanism.
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Purified SSIII lost activity after brief incubation at relatively low temperatures.