A glutathione-S-transferase (TuGSTd05) associated with acaricide resistance in Tetranychus urticae directly metabolizes the complex II inhibitor cyflumetofen
TuGSTd05 is over-expressed in a cyflumetofen-resistant Tetranychus urticae strain. Cyflumetofen de-esterified metabolite is a strong inhibitor of GST activity in vitro. Recombinant enzyme catalyzes the conjugation of GSH to cyflumetofen/de-esterified metabolite. Site of attack, metabolite structure and key amino acids involved in the interaction were identified.