Structure and antioxidant activity of polysaccharide POJ-U1a extracted by ultrasound from Ophiopogon japonicus

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• 作者：Xiao-mei Wang^a ; Run-guang Sun^a ; ^{runguangsun@163.com} ; Jing Zhang^b ; Ying-ying Chen^a ; Na-nv Liu^a
• 关键词：Polysaccharide structure ; Ultrasonic extraction ; Ophiopogon japonicus ; Antioxidant activity
• 刊名：Fitoterapia
• 出版年：2012
• 出版时间：December, 2012
• 年：2012
• 卷：83
• 期：8
• 页码：1576-1584
• 全文大小：802 K

文摘

An ultrasonic technique was employed to extract polysaccharides from Ophiopogon japonicus. The ultrasonic extracted polysaccharides (POJ-U) were purified, and POJ-U1a (a homogeneous fraction) was obtained. The structural characteristics of POJ-U1a were investigated by infrared spectra, gas chromatography, high performance liquid chromatography, periodate oxidation, Smith degradation, methylation analysis, gas chromatography-mass spectrometry and nuclear magnetic resonance spectroscopy. The results indicated that the relative molecular weight of POJ-U1a was 4.02 ¡Á 10³ Da. POJ-U1a was an ¦Á-configuration polysaccharide with a highly branched structure, and consisted of pyranoside and funanside. The backbone of POJ-U1a consisted of 1,6-¦Á-d-glucopyranose and 1,3,6-¦Á-d-glucofuranose in the molar ratio of 7:3, while the branched chains were mainly composed of 1,3-¦Á-d-glucopyranose and 1-¦Á-d-glucopyranose in the molar ratio of 1:3. The branched structure of POJ-U1a was proved intuitively by AFM. Significant antioxidant activity of POJ-U1a has been proved as shown by its DPPH radical scavenging, hydrogen radical scavenging and superoxide anion scavenging activities, which indicated that POJ-U1a showed strong antioxidant activity.