Ferulic acid decreases cell viability and colony formation while inhibiting migration of MIA PaCa-2 human pancreatic cancer cells in vitro

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• 作者：Umut Fahrioğlu^a ; ^{umutfahrioglu@gmail.com" class="auth_mail" title="E-mail the corresponding author} ; ^{umut.fahrioglu@neu.edu.tr" class="auth_mail" title="E-mail the corresponding author} ; Yavuz Dodurga^b ; Levent Elmas^b ; M&uuml ; cahit Seç ; me^b
• 关键词：FA ; Ferulic acid ; CCND1 ; Cyclin D1 ; CDK4 ; Cyclin Dependent Kinase 4 ; CDK6 ; Cyclin Dependent Kinase 6 ; RB ; Retinablastoma gene ; BCL-XL ; B-cell lymphoma-extra large ; BID ; BH3 Interacting Domain Death Agonist ; DR4 ; HLA-DR4 ; DR5 ; Death receptor 5 ; FADD ; Fas-Associated protein with Death Domain ; TRADD ; Tumor Necrosis Factor Receptor Type 1 Associated Death Domain Protein ; PARP ; poly ADP ribose polymerase ; APAF ; Apoptotic protease activating factor 1 ; PTEN ; Phosphatase and tensin homolog ; PUMA ; p53 up
• 刊名：Gene
• 出版年：2016
• 出版时间：15 January 2016
• 年：2016
• 卷：576
• 期：1
• 页码：476-482
• 全文大小：940 K

文摘

Novel and combinatorial treatment methods are becoming sought after entities in cancer treatment and these treatments are even more valuable for pancreatic cancer. The scientists are always on the lookout for new chemicals to help them in their fight against cancer. In this study, we examine the effects of ferulic acid (FA), a phenolic compound, on gene expression, viability, colony formation and migration/invasion in the cultured MIA PaCa-2 human pancreatic cancer cell. Cytotoxic effects of FA were determined by using trypan blue dye exclusion test and Cell TiterGlo (CTG) assay. IC₅₀ dose in MIA PaCa-2 cells was detected as 500 μM/ml at the 72nd hour. Expression profiles of certain cell cycle and apoptosis genes such as CCND1 (cyclin D1),CDK4, CDK6, RB, p21, p16, p53, caspase-3, caspase-9, caspase-8, caspase-10, Bcl-2, BCL-XL,BID, DR4,DR5,FADD,TRADD,PARP, APAF, Bax, Akt, PTEN, PUMA, NOXA, MMP2, MMP9, TIMP1 and TIMP2 were determined by real-time PCR. The effect of FA on cell viability was determined by CellTiter-Glo® Luminescent Cell Viability Assay. Additionally, effects of FA on colony formation and invasion were also investigated. It was observed that FA caused a significant decrease in the expression of CCND1, CDK 4/6, Bcl2 and caspase 8 and 10 in the MIA PaCa-2 cells while causing an increase in the expression of p53, Bax, PTEN caspase 3 and 9. FA was observed to decrease colony formation while inhibiting cell invasion and migration as observed by the BioCoat Matrigel Invasion Chamber guide and colony formation assays. In conclusion, FA is thought to behave as an anti-cancer agent by affecting cell cycle, apoptotic, invasion and colony formation behavior of MIA PaCa-2 cells. Therefore, FA is placed as a strong candidate for further studies aimed at finding a better, more effective treatment approach for pancreatic cancer.