Small GTPase Rab39A interacts with UACA and regulates the retinoic acid-induced neurite morphology of Neuro2A cells

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• 作者：Yasunori Mori ; Takahide Matsui ; Daisuke Omote ; Mitsunori Fukuda ; ^{nori@m.tohoku.ac.jp}
• 关键词：aa ; amino acid residues ; CA/CN ; constitutive active/negative ; CC ; coiled-coil ; EGFP ; enhanced green fluorescent protein ; RA ; retinoic acid ; shRNA ; short hairpin RNA ; SR ; shRNA-resistant ; mStr ; monomeric Strawberry ; UACA ; uveal autoantigen with coiled-coil
• 刊名：Biochemical and Biophysical Research Communications
• 出版年：2013
• 出版时间：24 May, 2013
• 年：2013
• 卷：435
• 期：1
• 页码：113-119
• 全文大小：1137 K

文摘

We screened for a Rab39-specific effector by performing a yeast two-hybrid assay with GTP-locked Rab39A/B as the bait and identified UACA (uveal autoantigen with coiled-coil domains and ankyrin repeats) as a specific Rab39A/B-binding protein. Deletion analysis revealed that a C-terminal coiled-coil domain of UACA functions as a GTP-dependent Rab39-binding domain. shRNA-mediated knockdown of endogenous Rab39A or UACA in mouse neuroblastoma Neuro2A cells resulted in a change in retinoic acid-induced neurite morphology from a multipolar morphology to a bipolar morphology. Taken together, these findings indicate that UACA functions as a Rab39A effector in the retinoic acid-induced differentiation of Neuro2A cells.