First-time studied pH and T effects on S. roseochromogenes 16α-hydroxylation of hydrocortisone.
Conversion at 26 °C and pH 6 led to a 95% 16α-hydroxyhydrocortisone on total product ratio.
Two-step substrate addition resulted suitable for 1.0 g/L hydrocortisone transformation.
Fed-batch fermentation improved transformation up to 0.807 g/L of 16α-hydroxyhydrocortisone.
Proposed reverse phase purification drove to 85% recovery of 16α-hydroxyhydrocortisone.