A single nucleotide polymorphism in the translation elongation factor 1伪 gene correlates with the ability to produce fumonisin in Japanese Fusarium fujikuroi

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• 作者：Haruhisa Suga^a ; ^{suga@gifu-u.ac.jp" class="auth_mail} ; Miha Kitajima^b ; Riku Nagumo^b ; Takao Tsukiboshi^c ; Ryuichi Uegaki^c ; Takashi Nakajima^d ; Masayo Kushiro^e ; Hiroyuki Nakagawa^e ; Masafumi Shimizu^b ; Koji Kageyama^f ; Mitsuro Hyakumachi^b
• 关键词：Fungi ; Gibberella fujikuroi ; Mycotoxin ; Rice pathogen ; Bakanae disease
• 刊名：Fungal Biology
• 出版年：April, 2014
• 年：2014
• 卷：118
• 期：4
• 页码：402-412
• 全文大小：649 K

文摘

PCR-RFLP based on the translation elongation factor 1伪 (TEF) gene was developed to identify Fusarium fujikuroi in the Fusarium (Gibberella) fujikuroi species complex. Ninety-three strains, most of which were obtained from various sources in Japan, were identified as F. fujikuroi and their capability to produce fumonisin was investigated using an in聽vitro assay. Fumonisin production was detected in 50 strains isolated from maize, strawberry, wheat, and rice, whereas it was undetectable in 43 strains derived from rice seeds and rice seedlings carrying the bakanae disease, and from unknown sources. A single nucleotide polymorphism in the TEF gene (T618G) correlated with the ability to synthesize fumonisin.