Simultaneous Determination of Eight Active Components in Liuwei Wuling Tablet Using HPLC

详细信息    查看全文

• 作者：He-rong Cui^a ; ^b ; ^&dagger ; ; ^{herongcui@outlook.com" class="auth_mail" title="E-mail the corresponding author} ; Gen-hua Xu^b ; ^&dagger ; ; ; Ming-quan Wu^b ; Wen-yan Jiang^b ; Jing-jing Han^b ; Jia-bo Wang^b ; Zhao-fang Bai^b ; ^{baizf2008@hotmail.com" class="auth_mail" title="E-mail the corresponding author} ; Guang-ming Cai^b ; ; Xiao-he Xiao^c ;
• 关键词：forsythoside A ; hepatoprotective ; Liuwei Wuling Tablet ; salidroside
• 刊名：Chinese Herbal Medicines
• 出版年：2016
• 出版时间：October 2016
• 年：2016
• 卷：8
• 期：4
• 页码：331-336
• 全文大小：607 K

文摘

To develop an effective method for simultaneous determination of eight major components in Liuwei Wuling Tablet (LWT).

Methods

Reversed phase HPLC method was used with variously improved conditions.

Results

Salidroside, forsythoside A, specnuezhenide, phillyrin, schisandrol A, schizandrol A, schizandrin A, and schizandrin B in LWT were successfully separated on a Kromasil 100-5-C₁₈ reverse phase column (250 mm × 4.6 mm, 5 μm) using acetonitrile-0.1% phosphoric acid (gradient elution) as mobile phase. The detection wavelength was 275 nm with flow rate of 1.0 mL/min, and the column temperature was maintained at 35 ^oC. The recovery rate of the method was within the range of 95.13%–104.56% and the precision (RSD) was less than 3% for all eight analytes. All the compounds showed good linearities (r² > 0.9980) in a relatively wide concentration range.

Conclusion

Simultaneous quantification of the multiple components by HPLC would be a better strategy for the quality evaluation of LWT.