Schisandrin B suppresses glioma cell metastasis mediated by inhibition of mTOR/MMP-9 signal pathway

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• 作者：Yan Jiang ; Qiuli Zhang ; Jinsuo Bao ; Chenghua Du ; ^{Du_chua@163.com" class="auth_mail" title="E-mail the corresponding author} ; Jian Wang ; Qiang Tong ; Chang Liu
• 关键词：Akt ; mTOR ; Cell invasion ; Cell migration ; PI3K
• 刊名：Biomedicine & Pharmacotherapy
• 出版年：2015
• 出版时间：August 2015
• 年：2015
• 卷：74
• 期：Complete
• 页码：77-82
• 全文大小：2317 K

文摘

Malignant glioma is the aggressive tumor in the brain and is characterized by high morbidity, high mortality. The main purpose of the present study was to investigate the therapeutic effects of Schisandrin B on glioma cells and preliminary explore the possible mechanism underlying anti-metastasis of Schisandrin B.

Methods

Two glioma cell lines, U251 and U87, were used in present study. The ability of metastasis of glioma cells was evaluated using transwell migration assay and invasion assay. Expression of Akt, mTOR, MMP-2 and MMP-9 was determined using Western blotting. Antagonist or agonist was used to activated or inactivated signal molecules.

Results

Schisandrin B suppressed cell migration and invasion in manner of dose dependent as well as inhibited expression of p-Akt, p-mTOR and MMP-9. Activation of PI3K by 740Y-P treatment leaded to upregulation of p-Akt, mTOR and MMP-9; inactivation of mTOR by Rapamycin treatment inhibited expression MMP-9 while activation of mTOR by l-Leucine treatment enhanced MMP-9 expression in Schisandrin B incubated cells. Anti-migration and invasion action of Schisandrin B was also reversed by mTOR activation.

Conclusion

Our findings demonstrate that Schisandrin B can suppress migration and invasion of glioma cell via PI3K/Akt-mTOR-MMP-9 signaling pathway.