High throughput gene complementation screening permits identification of a mammalian mitochondrial protein synthesis (ρ−) mutant
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- 作者：Prasanth Potluria ; Vincent Procacciob ; Immo E. Schefflerc ; Douglas C. Wallacea ; wallaced1@email.chop.edu" class="auth_mail" title="E-mail the corresponding author
- 关键词：Mitochondrial diseases ; Complementation cloning ; OXPHOS ; Mitochondrial ribosomes ; bS6m
- 刊名：Biochimica et Biophysica Acta (BBA) - Bioenergetics
- 出版年：2016
- 出版时间：August 2016
- 年：2016
- 卷：1857
- 期：8
- 页码：1336-1343
- 全文大小：1316 K
文摘
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Functional complementation is a viable experimental strategy in determining nuclear mutations affecting mitochondria.
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cDNA expression library screening identifies the defective bS6m gene in a previously un-characterized mutant cell line.
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Our approach is a robust alternative for cloning unknown genes with mitochondrial function.