A novel allelopathic substance, 13-epi-orthosiphol N, in Orthosiphon stamineus

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• 作者：Hisashi Kato-Noguchi^a ; ^{hisashi@ag.kagawa-u.ac.jp} ; Naoko Hamada^a ; Maho Morita^b ; Kiyotake Suenaga^b
• 关键词：Allelopathy ; Biological activity ; Growth inhibitor ; Lamiaceae ; Orthosiphon stamineus
• 刊名：Journal of Plant Physiology
• 出版年：2013
• 出版时间：1 January, 2013
• 年：2013
• 卷：170
• 期：1
• 页码：1-5
• 全文大小：534 K

文摘

Orthosiphon stamineus (Java tea) has been widely used as traditional herb and several bioactive compounds against animal cells have been isolated. However, no bioactive compound against plants has been reported. Therefore, we investigated possible allelopathic properties and substances in O. stamineus. Aqueous methanol extracts of O. stamineus inhibited root and hypocotyl growth of cress (Lepidium sativum) and lettuce (Lactuca sativa) seedlings. Increasing the extract concentration increased the inhibition, which suggests that O. stamineus may have allelopathic properties. When the extract was divided into an ethyl acetate and an aqueous fraction, the ethyl acetate fraction showed the stronger inhibitory effect. Thus, the ethyl acetate phase was further purified, and the main allelopathic substance was isolated and identified as 13-epi-orthosiphol N, a novel compound, by spectral data. 13-epi-Orthosiphol N inhibited root and hypocotyl growth of cress and lettuce at concentrations greater than 10 ¦Ìmol/L. The concentrations required for 50 % inhibition ranged from 41 to 102 ¦Ìmol/L. These results suggest that 13-epi-orthosiphol N may be an allelochemical and main contributor to the growth inhibitory effect of O. stamineus and may have potential as a template for the development of new plant control substances.