A new extracellular thermo-solvent-stable lipase from Burkholderia ubonensis SL-4: Identification, characterization and application for biodiesel production
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- 作者：Wenjuan Yang ; Yaojia He ; Li Xu ; Houjin Zhang ; Yunjun Yan ; yanyunjun@hust.edu.cn" class="auth_mail" title="E-mail the corresponding author
- 关键词：Burkholderia ubonensis ; Purification ; Organic solvent-tolerant lipase ; Biodiesel ; Solvent-free system
- 刊名：Journal of Molecular Catalysis B: Enzymatic
- 出版年：2016
- 出版时间：April 2016
- 年：2016
- 卷：126
- 期：Complete
- 页码：76-89
- 全文大小：4235 K
文摘
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A new lipase SL-4 of Burkholderia ubonensis SL-4 was more stable at 50 °C and pH 8.5.
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Lipase SL-4 was activated by Ca2+ or Mn2+, but inhibited by EDTA, PMSF, DTT and β-ME.
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Lipase SL-4 had non-ionic detergent- and organic solvent-stability.
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Liquid lipase SL-4 could catalyze soybean oil to biodiesel at a yield rate of 92.24%.