Critical Assessment of Various Techniques for the Extraction of Carotenoids and Co-enzyme Q10 from the Thraustochytrid Strain ONC-T18
详细信息 查看全文
- 作者：Roberto E. Armenta ; Adam Burja ; Helia Radianingtyas ; Colin J. Barrow
- 刊名：Journal of Agricultural and Food Chemistry
- 出版年：2006
- 出版时间：December 27, 2006
- 年：2006
- 卷：54
- 期：26
- 页码：9752 - 9758
- 全文大小：182K
- 年卷期：v.54,no.26(December 27, 2006)
- ISSN：1520-5118
文摘
A variety of techniques for extracting carotenoids from the marine Thraustochytrium sp. ONC-T18was compared. Specifically, the organic solvents acetone, ethyl acetate, and petroleum ether weretested, along with direct and indirect ultrasonic assisted extraction (probe vs bath) methods.Techniques that used petroleum ether/acetone/water (15:75:10, v/v/v) with 3 h of agitation, or 5 minin an ultrasonic bath, produced the highest extraction yields of total carotenoids (29-30.5 
g g-1).Concentrations up to 11.5 g g-1 of canthaxanthin and 17.5 g g-1 of 
-carotene were detected inextracts stored for 6 weeks. Astaxanthin and echinenone were also detected as minor compounds.Extracts with and without antioxidants showed similar carotenoid concentration profiles. However,total carotenoid concentrations were approximately 8% higher when antioxidants were used. Finally,an easy-to-perform and inexpensive method to detect co-enzymes in ONC-T18 was also developedusing silica gel TLC plates. Five percent methanol in toluene as a mobile phase consistently elutedco-enzyme Q10 standards and could separate the co-enzyme fractions present in ONC-T18.
g g-1).Concentrations up to 11.5 g g-1 of canthaxanthin and 17.5 g g-1 of -carotene were detected inextracts stored for 6 weeks. Astaxanthin and echinenone were also detected as minor compounds.Extracts with and without antioxidants showed similar carotenoid concentration profiles. However,total carotenoid concentrations were approximately 8% higher when antioxidants were used. Finally,an easy-to-perform and inexpensive method to detect co-enzymes in ONC-T18 was also developedusing silica gel TLC plates. Five percent methanol in toluene as a mobile phase consistently elutedco-enzyme Q10 standards and could separate the co-enzyme fractions present in ONC-T18.