New Covalent Capture Probes for Imaging and Therapy, Based on a Combination of Binding Affinity and Disulfide Bond Formation

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• 作者：Tolulope A. Aweda ; Vahid Eskandari ; David L. Kukis ; David L. Boucher ; Bernadette V. Marquez ; Heather E. Beck ; Gregory S. Mitchell ; Simon R. Cherry ; Claude F. Meares
• 刊名：Bioconjugate Chemistry
• 出版年：2011
• 出版时间：August 17, 2011
• 年：2011
• 卷：22
• 期：8
• 页码：1479-1483
• 全文大小：852K
• 年卷期：v.22,no.8(August 17, 2011)
• ISSN：1520-4812

文摘

We describe the synthesis and development of new reactive DOTA-metal complexes for covalently targeting engineered receptors in vivo, which have superior tumor uptake and clearance properties for biomedical applications. These probes are found to clear efficiently through the kidneys and minimally through other routes, but bind persistently in the tumor target. We also explore the new technique of Cerenkov luminescence imaging to optically monitor radiolabeled probe distribution and kinetics in vivo. Cerenkov luminescence imaging uniquely enables sensitive noninvasive in vivo imaging of a 尾^{鈥?/sup> emitter such as 90Y with an optical imager.}