Neutrophil Chemotaxis within a Competing Gradient of Chemoattractants
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- 作者：Donghyuk Kim ; Christy L. Haynes
- 刊名：Analytical Chemistry
- 出版年：2012
- 出版时间：July 17, 2012
- 年：2012
- 卷：84
- 期：14
- 页码：6070-6078
- 全文大小：393K
- 年卷期：v.84,no.14(July 17, 2012)
- ISSN：1520-6882
文摘
The dynamics of neutrophil chemotaxis under competing chemoattractant gradients was studied using a microfluidic platform. This microfluidic platform, which establishes a stable and dynamic gradient of chemoattractants across a cell culture chamber, enabled the investigation of human neutrophil migration patterns in the presences of four different chemoattractants (leukotriene B4, chemokine C鈥揦鈥揅 motif ligands 2 and 8, and fMLP) and competing gradients of all pairwise combinations. The migration patterns for individual cells were tracked and quantitatively analyzed, and the results suggest a hierarchy among these chemoattractants of fMLP > CXCL8 > CXCL2 > leukotriene B4. In all conditions, over 60% of neutrophils exposed to a competing gradient move toward the stronger signal though the weaker chemoattractant still influences neutrophil motility. These results yield insight about how each chemoattractant contributes to overall neutrophil chemotaxis within complex physiological environments.