Paired Design of dCas9 as a Systematic Platform for the Detection of Featured Nucleic Acid Sequences in Pathogenic Strains
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- 作者：Yihao Zhang ; Long Qian ; Weijia Wei ; Yu Wang ; Beining Wang ; Pingping Lin ; Wenchao Liu ; Luze Xu ; Xiang Li ; Dongming Liu ; Sida Cheng ; Jiaofeng Li ; Yixuan Ye ; Hang Li ; Xiaohan Zhang ; Yiming Dong ; Xuejin Zhao ; Cuihua Liu ; Haoqian M. Zhang ; Qi Ouyang ; Chunbo Lou
- 刊名：ACS Synthetic Biology
- 出版年：2017
- 出版时间：February 17, 2017
- 年：2017
- 卷：6
- 期：2
- 页码：211-216
- 全文大小：380K
- ISSN：2161-5063
文摘
We developed an <i>in vitroi> DNA detection system using a pair of dCas9 proteins linked to split halves of luciferase. Luminescence was induced upon colocalization of the reporter pair to a ∼44 bp target sequence defined by sgRNAs. We used the system to detect <i>Mycobacterium tuberculosisi> DNA with high specificity and sensitivity. The reprogrammability of dCas9 was further leveraged in an array design that accesses sequence information across the entire genome.