Investigation of pH-Induced Protein Conformation Changes by Nanomechanical Deflection

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• 作者：Garima Thakur ; Keren Jiang ; Dongkyu Lee ; Kovur Prashanthi ; Seonghwan Kim ; Thomas Thundat
• 刊名：Langmuir
• 出版年：2014
• 出版时间：March 4, 2014
• 年：2014
• 卷：30
• 期：8
• 页码：2109-2116
• 全文大小：400K
• 年卷期：v.30,no.8(March 4, 2014)
• ISSN：1520-5827

文摘

Broad-spectrum biosensing technologies examine sensor signals using biomarkers, such as proteins, DNA, antibodies, specific cells, and macromolecules, based on direct- or indirect-conformational changes. Here, we have investigated the pH-dependent conformational isomerization of human serum albumin (HSA) using microcantilevers as a sensing platform. Native and denatured proteins were immobilized on cantilever surfaces to understand the effect of pH on conformational changes of the protein with respect to the coupling ligand. Our results show that protonation and deprotonation of amino acid residues on proteins play a significant role in generating charge-induced cantilever deflection. Surface plasmon resonance (SPR) was employed as a complementary technique to validate the results.