Functionalization of Luminescent Aminated Particles for Facile Bioconjugation

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• 作者：Anne-Charlotte Faure ; Céline Hoffmann ; Rana Bazzi ; Fabrice Goubard ; Emmanuel Pauthe ; Christophe A. Marquette ; Loïc J. Blum ; Pascal Perriat ; Stéphane Roux ; Olivier Tillement.
• 刊名：ACS Nano
• 出版年：2008
• 出版时间：November 25, 2008
• 年：2008
• 卷：2
• 期：11
• 页码：2273-2282
• 年卷期：0
• ISSN：1936-086X

文摘

For labeling proteins (streptavidin and fibronectin) by luminescent aminated nanoparticles, an interesting strategy that requires neither activation nor chemical pre- or post-treatment was explored. Because biomolecules are easily rendered luminescent after reaction with organic dyes carrying isothiocyanate moiety, phenylene diisothiocyanate (DITC) was used for covalently binding proteins onto luminescent hybrid gadolinium oxide nanoparticles whose ability to combine imaging and therapy was recently demonstrated.