In Vivo Targeting and Imaging of Tumor Vasculature with Radiolabeled, Antibody-Conjugated Nanographene
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- 作者：Hao Hong ; Kai Yang ; Yin Zhang ; Jonathan W. Engle ; Liangzhu Feng ; Yunan Yang ; Tapas R. Nayak ; Shreya Goel ; Jero Bean ; Charles P. Theuer ; Todd E. Barnhart ; Zhuang Liu ; Weibo Cai
- 刊名：ACS Nano
- 出版年：2012
- 出版时间：March 27, 2012
- 年：2012
- 卷：6
- 期：3
- 页码：2361-2370
- 全文大小：1098K
- 年卷期：v.6,no.3(March 27, 2012)
- ISSN：1936-086X
文摘
Herein we demonstrate that nanographene can be specifically directed to the tumor neovasculature in vivo through targeting of CD105 (i.e., endoglin), a vascular marker for tumor angiogenesis. The covalently functionalized nanographene oxide (GO) exhibited excellent stability and target specificity. Pharmacokinetics and tumor targeting efficacy of the GO conjugates were investigated with serial noninvasive positron emission tomography imaging and biodistribution studies, which were validated by in vitro, in vivo, and ex vivo experiments. The incorporation of an active targeting ligand (TRC105, a monoclonal antibody that binds to CD105) led to significantly improved tumor uptake of functionalized GO, which was specific for the neovasculature with little extravasation, warranting future investigation of these GO conjugates for cancer-targeted drug delivery and/or photothermal therapy to enhance therapeutic efficacy. Since poor extravasation is a major hurdle for nanomaterial-based tumor targeting in vivo, this study also establishes CD105 as a promising vascular target for future cancer nanomedicine.