Chitinase Activity on Amorphous Chitin Thin Films: A Quartz Crystal Microbalance with Dissipation Monitoring and Atomic Force Microscopy Study

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• 作者：Chao Wang ; Joshua D. Kittle ; Chen Qian ; Maren Roman ; Alan R. Esker
• 刊名：Biomacromolecules
• 出版年：2013
• 出版时间：August 12, 2013
• 年：2013
• 卷：14
• 期：8
• 页码：2622-2628
• 全文大小：463K
• 年卷期：v.14,no.8(August 12, 2013)
• ISSN：1526-4602

文摘

Chitinases are widely distributed in nature and have wide-ranging pharmaceutical and biotechnological applications. This work highlights a real-time and label-free method to assay Chitinase activity via a quartz crystal microbalance with dissipation monitoring (QCM-D) and atomic force microscopy (AFM). The chitin substrate was prepared by spincoating a trimethylsilyl chitin solution onto a silica substrate, followed by regeneration to amorphous chitin (RChi). The QCM-D and AFM results clearly showed that the hydrolysis rate of RChi films increased as Chitinase (from Streptomyces griseus) concentrations increased, and the optimal temperature and pH for Chitinase activity were around 37 掳C and 6鈥?, respectively. The Chitinase showed greater activity on chitin substrates, having a high degree of acetylation, than on chitosan substrates, having a low degree of acetylation.