Synthesis and Characterization of a Carbon Nanotube−Dendron Series for Efficient siRNA Delivery

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• 作者：M. Antonia Herrero ; Francesca M. Toma ; Khuloud T. Al-Jamal ; Kostas Kostarelos ; Alberto Bianco ; Tatiana Da Ros ; Fouzia Bano ; Loredana Casalis ; Giacinto Scoles ; Maurizio Prato
• 刊名：Journal of the American Chemical Society
• 出版年：2009
• 出版时间：July 22, 2009
• 年：2009
• 卷：131
• 期：28
• 页码：9843-9848
• 全文大小：308K
• 年卷期：v.131,no.28(July 22, 2009)
• ISSN：1520-5126

文摘

A new series of dendron-functionalized multiwalled carbon nanotube (MWNT) derivatives, characterized by the presence of numerous positively charged tetraalkyl ammonium salts at the periphery of the dendron, has been synthesized. The positive charges on the MWNT surface, coupled with the unique ability of carbon nanotubes (CNTs) to penetrate cell membranes, make the new derivatives potentially ideal vectors for siRNA delivery. Using a fluorescently labeled, noncoding siRNA sequence, we demonstrate that cytoplasmic delivery of the nucleic acid is remarkably increased throughout the different dendron generations. The work reported here highlights the fact that dendron-functionalized CNTs can be rationally designed as efficient carriers of siRNA that can eventually lead to gene silencing.