Proteomic Analyses of Zebra Finch Optic Tectum and Comparative Histochemistry

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• 作者：Stephanie Sloley ; Shannon Smith ; Sonia Gandhi ; Jennifer A. Caldwell Busby ; Sarah London ; Harald Luksch ; David F. Clayton ; Sanjoy K. Bhattacharya
• 刊名：Journal of Proteome Research
• 出版年：2007
• 出版时间：June 2007
• 年：2007
• 卷：6
• 期：6
• 页码：2341 - 2350
• 全文大小：437K
• 年卷期：v.6,no.6(June 2007)
• ISSN：1535-3907

文摘

Proteomic analyses of zebra finch (Taeniopygia guttata) optic tectum resulted in identification of 176proteins. In the Swissprot database, only 52 proteins were identified as bird homologs and only 71proteins were identified in songbird transcriptome databases, reflecting a lack of completeness in theT. guttata genomic sequence. Analysis in Kyoto encyclopedia of genes and genome (KEGG) pathwaydatabase found that identified proteins most frequently belong to glucose, pyruvate, glyoxylate,dicarboxylate, alanine, and aspartate metabolism pathways. A number of identified proteins have beenpreviously reported to exist in the avian optic tectum. The immunohistochemical localization of selectedproteins showed their distribution in similar laminae of the owl (Tyto alba) and chicken (Gallus gallus)tectum. Immunohistochemical analysis of identified proteins can provide clues about cell types andcircuit layout of the avian optic tectum in general. As the optic tectum of nonmammals is homologousto the superior colliculus of mammals, the analysis of the tectal and collicular proteome may provideclues about conserved cell and circuit layout, circuit function, and evolution.