Validation and Interpretation of CALUX as a Tool for the Estimation of Dioxin-Like Activity in Marine Biological Matrixes

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• 作者：Isabelle Windal ; Nathalie Van Wouwe ; Gauthier Eppe ; Cé ; line Xhrouet ; Virginie Debacker ; Willy Baeyens ; Edwin De Pauw ; and Leo Goeyens
• 刊名：Environmental Science & Technology
• 出版年：2005
• 出版时间：March 15, 2005
• 年：2005
• 卷：39
• 期：6
• 页码：1741 - 1748
• 全文大小：198K
• 年卷期：v.39,no.6(March 15, 2005)
• ISSN：1520-5851

文摘

Among the different analytical tools proposed as analternative to the very expensive gas chromatography high-resolution mass spectrometry (GC-HRMS) analyses ofpolychlorodibenzo-p-dioxin and polychlorodibenzofurans,Chemically Activated LUciferase gene eXpression (CALUX)in vitro cell bioassay is very promising. It allows theanalyses of a high number of samples since it is relativelyfast, inexpensive, and sensitive. However, this techniqueis not yet widely applied for screening or environmentalmonitoring. The main reasons are probably the lackof validation and the difficulty in interpreting the globalbiological response of the bioassay. In this paper, the strictquality control criteria set up for the validation of CALUXare described. The validation has shown good repeatability(relative standard deviation (RSD) = 9%) and good within-lab reproducibility (RSD = 15%) of the results. Thequantification limit, in the conditions applied in this paper,is 1.25 pg CALUX-TEQ/g fat. Comparison of CALUX and GC-HRMS analysis was made for various marine matrixes (fishes,mussels, starfishes, sea birds, and marine mammals).Good correlations are usually observed, but there aresystematic differences between the results. Attempts aremade to identify the origin of the discrepancy betweenthe two methods.