Dabigatran and Dabigatran Ethyl Ester: Potent Inhibitors of Ribosyldihydronicotinamide Dehydrogenase (NQO2)

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• 作者：Simon Michaelis ; Anett Marais ; Anna K. Schrey ; Olivia Y. Graebner ; Cornelia Schaudt ; Michael Sefkow ; Friedrich Kroll ; Mathias Dreger ; Mirko Glinski ; Hubert Koester ; Rainer Metternich ; Jenny J. Fischer
• 刊名：Journal of Medicinal Chemistry
• 出版年：2012
• 出版时间：April 26, 2012
• 年：2012
• 卷：55
• 期：8
• 页码：3934-3944
• 全文大小：437K
• 年卷期：v.55,no.8(April 26, 2012)
• ISSN：1520-4804

文摘

Recent studies have revealed that compounds believed to be highly selective frequently address multiple target proteins. We investigated the protein interaction profile of the widely prescribed thrombin inhibitor dabigatran (1), resulting in the identification and subsequent characterization of an additional target enzyme. Our findings are based on an unbiased functional proteomics approach called capture compound mass spectrometry (CCMS) and were confirmed by independent biological assays. 1 was shown to specifically bind ribosyldihydronicotinamide dehydrogenase (NQO2), a detoxification oxidoreductase. Molecular dockings predicted and biological experiments confirmed that dabigatran ethyl ester (2) inhibits NQO2 even more effectively than the parent 1 itself. Our data show that 1 and 2 are inhibitors of NQO2, thereby revealing a possible new aspect in the mode of action of 1. We present a workflow employing chemical proteomics, molecular modeling, and functional assays by which a compound鈥檚 protein-interaction profile can be determined and used to tune the binding affinity.