Mannose-Substituted Conjugated Polyelectrolyte and Oligomer as an Intelligent Energy Transfer Pair for Label-Free Visual Detection of Concanavalin A

详细信息    查看全文

• 作者：Kan-Yi Pu ; Jianbing Shi ; Lihua Wang ; Liping Cai ; Guan Wang ; Bin Liu
• 刊名：Macromolecules
• 出版年：2010
• 出版时间：December 14, 2010
• 年：2010
• 卷：43
• 期：23
• 页码：9690-9697
• 全文大小：964K
• 年卷期：v.43,no.23(December 14, 2010)
• ISSN：1520-5835

文摘

Mannose-substituted blue-fluorescent cationic conjugated polyelectrolyte (P1) and yellow-fluorescent neutral conjugated oligomer (6) are synthesized via the combination of Suzuki coupling polymerization and click chemistry. The spectral overlap between the emission of P1 and the absorption of 6 allows one to blend them to form an intelligent fluorescence resonance energy transfer (FRET) pair for multicolor protein sensing. Despite the nonspecific-interaction-induced perturbation in the polymer emission, strong specific binding between mannose and Concanavalin A (ConA) is able to result in protein-selective FRET from P1 to 6. The fluorescence of 6/P1 blend in the presence of ConA is dominated by yellow emission, while it remains blue in the presence of other six nonspecific proteins. The 6/P1 blend thus serves as a multicolor bicomponent FRET probe for label-free visual detection of ConA in a high-contrast and convenient manner. In addition, its linear ratiometric fluorescence response toward ConA enables effective protein quantification with a low limit of detection of 1.5 nM. This study demonstrates the importance of molecular engineering in conjugated polyelectrolyte based label-free chemical and biological sensing.