Chemical Mimicry: Hierarchical 1D TiO2@ZrO2 Core鈭扴hell Structures Reminiscent of Sponge Spicules by the Synergistic Effect of Silicatein-伪 and Silintaphin-1

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• 作者：Rute Andre虂 ; Muhammad Nawaz Tahir ; Thorben Link ; Florian D. Jochum ; Ute Kolb ; Patrick Theato ; Ru虉diger Berger ; Matthias Wiens ; Heinz-Christoph Schro虉der ; Werner E. G. Mu虉ller ; Wolfgang Tremel
• 刊名：Langmuir
• 出版年：2011
• 出版时间：May 3, 2011
• 年：2011
• 卷：27
• 期：9
• 页码：5464-5471
• 全文大小：991K
• 年卷期：v.27,no.9(May 3, 2011)
• ISSN：1520-5827

文摘

In nature, mineralization of hard tissues occurs due to the synergistic effect of components present in the organic matrix of these tissues, with templating and catalytic effects. In Suberites domuncula, a well-studied example of the class of demosponges, silica formation is mediated and templated by an axial proteinaceous filament with silicatein-伪, one of the main components. But so far, the effect of other organic constituents from the proteinaceous filament on the catalytic effect of silicatein-伪 has not been studied in detail. Here we describe the synthesis of core鈭抯hell TiOb>2b>@SiOb>2b> and TiOb>2b>@ZrOb>2b> nanofibers via grafting of silicatein-伪 onto a TiOb>2b> nanowire backbone followed by a coassembly of silintaphin-1 through its specifically interacting domains. We show for the first time a linker-free, one-step funtionalization of metal oxides with silicatein-伪 using glutamate tag. In the presence of silintaphin-1 silicatein-伪 facilitates the formation of a dense layer of SiOb>2b> or ZrOb>2b> on the TiOb>2b>@protein backbone template. The immobilization of silicatein-伪 onto TiOb>2b> probes was characterized by atomic force microscopy (AFM), optical light microscopy, and high-resolution transmission electron microscopy (HRTEM). The coassembly of silicatein-伪 and silintaphin-1 may contribute to biomimetic approaches that pursue a controlled formation of patterned biosilica-based biomaterials.