A Label-Free, Electrochemical SERS-Based Assay for Detection of DNA Hybridization and Discrimination of Mutations

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• 作者：Robert P. Johnson ; James A. Richardson ; Tom Brown ; Philip N. Bartlett
• 刊名：The Journal of the American Chemical Society
• 出版年：2012
• 出版时间：August 29, 2012
• 年：2012
• 卷：134
• 期：34
• 页码：14099-14107
• 全文大小：492K
• 年卷期：v.134,no.34(August 29, 2012)
• ISSN：1520-5126

文摘

A label-free, surface-enhanced Raman spectroscopy-based assay for detecting DNA hybridization at an electrode surface and for distinguishing between mutations in DNA is demonstrated. Surface-immobilized DNA is exposed to a binding agent that is selective for dsDNA and acts as a reporter molecule. Upon application of a negative potential, the dsDNA denatures into its constituent strands, and the changes in the spectra of the reporter molecule are monitored. This method has been used to distinguish between a wild-type, 1653C/T single-point mutation and 螖F508 triplet deletion in the CFTR gene. The use of dsDNA-selective binding agents as reporter molecules in a discrimination assay removes the burden of synthetically modifying the target to be detected, while retaining flexibility in the choice of the reporter molecule.