Smectite Clays as Solid Supports for Immobilization of β-Glucosidase: Synthesis, Characterization, and Biochemical Properties
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- 作者：Evangelia Serefoglou ; Kiriaki Litina ; Dimitrios Gournis ; Emmanuel Kalogeris ; Aikaterini A. Tzialla ; Ioannis V. Pavlidis ; Haralambos Stamatis ; Enrico Maccallini ; Monika Lubomska ; Petra Rudolf
- 刊名：Chemistry of Materials
- 出版年：2008
- 出版时间：June 24, 2008
- 年：2008
- 卷：20
- 期：12
- 页码：4106 - 4115
- 全文大小：198K
- 年卷期：v.20,no.12(June 24, 2008)
- ISSN：1520-5002
文摘
Nanomaterials as solid supports can improve the efficiency of immobilized enzymes by reducing diffusional limitation as well as by increasing the surface area per mass unit and therefore improving enzyme loading. In this work, β-glucosidase from almonds was immobilized on two smectite nanoclays. The resulting hybrid biocatalysts were characterized by a combination of powder X-ray diffraction, X-ray photoelectron spectroscopy, thermogravimetric analysis, differential thermal analysis, and infrared spectroscopy. Biochemical studies showed an improved thermostability of the immobilized enzyme as well as enhanced performance at higher temperatures and in a wider pH range.