In Vivo Imaging of Activated Endothelium Using an Anti-VCAM-1 Magnetooptical Probe

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• 作者：Andrew Tsourkas ; Vivek R. Shinde-Patil ; Kimberly A. Kelly ; Pratik Patel ; Allison Wolley ; Jennifer R. Allport ; and Ralph Weissleder
• 刊名：Bioconjugate Chemistry
• 出版年：2005
• 出版时间：May 2005
• 年：2005
• 卷：16
• 期：3
• 页码：576 - 581
• 全文大小：420K
• 年卷期：v.16,no.3(May 2005)
• ISSN：1520-4812

文摘

It has been suggested that vascular cell adhesion molecule-1 (VCAM-1) could serve as an early markerfor inflammation of the endothelium. The ability to noninvasively image VCAM-1 could thus be auseful tool to diagnose a number of inflammatory diseases at early stages. Here we demonstrate thatmagnetooptical nanoparticles conjugated to anti-VCAM-1 antibodies can be used to specifically detectVCAM-1 expression on endothelial cells in culture and in vivo. Elevated VCAM-1 expression wasdetected on cultured murine heart endothelial cells by both fluorescence and magnetic resonance,while only basal expression levels were detected on murine dermal endothelial cells. Intravitalmicroscopy of a murine inflammatory model injected with the VCAM-1 targeted nanoparticles revealedspecific labeling of the activated endothelium, with labeling kinetics yielding a maximum vessel wallsignal 6 h after injection. In contrast, nontargeted nanoparticles did not exhibit any specific labelingof the endothelium. These studies suggest that the developed nanoparticle would be useful for MRand optical detection of activated endothelium.