Single-Step Nanoplasmonic VEGF165 Aptasensor for Early Cancer Diagnosis

详细信息    查看全文

• 作者：Hansang Cho ; Erh-Chia Yeh ; Raghu Sinha ; Ted A. Laurence ; Jane P. Bearinger ; Luke P. Lee
• 刊名：ACS Nano
• 出版年：2012
• 出版时间：September 25, 2012
• 年：2012
• 卷：6
• 期：9
• 页码：7607-7614
• 全文大小：444K
• 年卷期：v.6,no.9(September 25, 2012)
• ISSN：1936-086X

文摘

Early cancer diagnosis is very important for the prevention or mitigation of metastasis. However, effective and efficient methods are needed to improve the diagnosis and assessment of cancer. Here, we report a single-step detection method using a nanoplasmonic aptamer sensor (aptasensor), targeting a vascular endothelial growth factor-165 (VEGF₁₆₅), a predominant biomarker of cancer angiogenesis. Our single-step detection is accomplished by (1) specific target recognition by an aptamer鈥搕arget molecule interaction and (2) direct readouts of the target recognition. The readout is achieved by inactivation of surface plasmon enhancement of fluorescent probes preattached to the aptamers. Our aptasensor provides the appropriate sensitivity for clinical diagnostics with a wide range of linear detection from 25 pg/mL to 25 渭g/mL (=from 1.25 pM to 1.25 渭M), high specificity for VEGF₁₆₅ against PDGF-BB, osteopontin (OPN), VEGF₁₂₁, NaCl, and temporal/thermal/biological stability. In experiments with 100% serum and saliva from clinical samples, readouts of the aptasensor and an ELISA for VEGF₁₆₅ show good agreement within the limit of the ELISA kit. We envision that our developed aptasensor holds utilities for point-of-care cancer prognostics by incorporating simplicity in detection, low-cost for test, and required small sample volumes.

Keywords:

aptamer; surface plasmonic resonance; optical biosensor; VEGF; early cancer diagnostics; prognostics; point-of-care diagnostics