Proteomic Analysis of Herbaspirillum seropedicae Cultivated in the Presence of Sugar Cane Extract
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- 作者：Fabio Aparecido Cordeiro ; Michelle Zibetti Tadra-Sfeir ; Luciano Fernandes Huergo ; F谩bio de Oliveira Pedrosa ; Rose Adele Monteiro ; Emanuel Maltempi de Souza
- 刊名：Journal of Proteome Research
- 出版年：2013
- 出版时间：March 1, 2013
- 年：2013
- 卷：12
- 期：3
- 页码：1142-1150
- 全文大小：321K
- 年卷期：v.12,no.3(March 1, 2013)
- ISSN：1535-3907
文摘
Bacterial endophytes of the genus Herbaspirillum colonize sugar cane and can promote plant growth. The molecular mechanisms that mediate plant鈥?H. seropedicae interaction are poorly understood. In this work, we used 2D-PAGE electrophoresis to identify H. seropedicae proteins differentially expressed at the log growth phase in the presence of sugar cane extract. The differentially expressed proteins were validated by RT qPCR. A total of 16 differential spots (1 exclusively expressed, 7 absent, 5 up- and 3 down-regulated) in the presence of 5% sugar cane extract were identified; thus the host extract is able to induce and repress specific genes of H. seropedicae. The differentially expressed proteins suggest that exposure to sugar cane extract induced metabolic changes and adaptations in H. seropedicae presumably in preparation to establish interaction with the plant.