High-Precision Tracking with Non-blinking Quantum Dots Resolves Nanoscale Vertical Displacement

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• 作者：Kyle Marchuk ; Yijun Guo ; Wei Sun ; Javier Vela ; Ning Fang
• 刊名：The Journal of the American Chemical Society
• 出版年：2012
• 出版时间：April 11, 2012
• 年：2012
• 卷：134
• 期：14
• 页码：6108-6111
• 全文大小：256K
• 年卷期：v.134,no.14(April 11, 2012)
• ISSN：1520-5126

文摘

Novel non-blinking quantum dots (NBQDs) were utilized in three-dimensional super-localization, high-precision tracking applications under an automated scanning-angle total internal reflection fluorescence microscope (SA-TIRFM). NBQDs were randomly attached to stationary microtubules along the radial axis under gliding assay conditions. By automatically scanning through a wide range of incident angles with different evanescent-field layer thicknesses, the fluorescence intensity decay curves were obtained. Fit with theoretical decay functions, the absolute vertical positions were determined with sub-10-nm localization precision. The emission intensity profile of the NBQDs attached to kinesin-propelled microtubules was used to resolve the self-rotation of gliding microtubules within a small vertical distance of 50 nm. We demonstrate the applicability of NBQDs in high-precision fluorescence imaging experiments.