Identification of Species in Animal Feedstuffs by Polymerase Chain Reaction-Restriction Fragment Length Polymorphism Analysis of Mitochondrial DNA

详细信息    查看全文

• 作者：Federica Bellagamba ; Vittorio M. Moretti ; Sergio Comincini ; and Franco Valfrè
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2001
• 出版时间：August 2001
• 年：2001
• 卷：49
• 期：8
• 页码：3775 - 3781
• 全文大小：341K
• 年卷期：v.49,no.8(August 2001)
• ISSN：1520-5118

文摘

Restriction site analysis of Polymerase Chain Reaction (PCR) products of cytochrome b mitochondrialDNA was applied to identify species in meat meal and animal feedstuffs. PCR was used to amplifya variable region of cytochrome b mitochondrial DNA gene. Species differentiation was determinedby digestion of the obtained 359 bp amplicon with restriction enzymes, which generated species-specific electrophoresis patterns; the sequencing of PCR products was used as confirming analysis.PCR-RFLP analysis revealed the presence of meat meal in animal feedstuffs and distinguishedspecies of interest. The results supported the application of the method in control measures whichshould be adopted for meat-meal-based animal feed, as suggested by EU law. As a technicalimprovement, to simplify the analysis, the number of enzymes presented in this study for thedetection of different species was smaller than others described in the literature; discriminationbetween ruminant and nonruminant species and between mammalian and poultry species waspossible with few digestions.Keywords: Species identification; meat meal; feedstuffs; PCR; cytochrome b gene